- 124 on Queen Hotel & Spa
- 2 Moon Cottage
- 627 on King Bed and Breakfast
- A Li'l Bit of Eden
- Abacot Hall Bed & Breakfast
- Antique Slumber Old Town Bed and Breakfast
- Apple Tree Historic B&B
- Arnica Bed & Breakfast
- As You Like It Bed and Breakfast
- Best Western Colonel Butler Inn
- Blairpen House Country Inn
- Blue Skies Bed & Breakfast
- Butler Creek House
- Canterbury Inn
- Casa Blanca Boutique Bed & Breakfast
- Cedar Gables Bed & Breakfast
- Cobblestone Bed and Breakfast
- Country Side Bed & Breakfast
- Darlington House Bed and Breakfast
- Downhome Bed & Breakfast
- Explorer House Bed & Breakfast
- Finlay House Bed and Breakfast Niagara - on - the - Lake
- Gatekeeper's Retreat
- Globetrotters B&B
- Graystone Bed & Breakfast
- Green Oaks B&B
- Greenview Manor Luxury Bed & Breakfast
- Harbour House
- Hilton Garden Inn Niagara-on-the-Lake
- Historic Lyons House
- Historic Wilson-Guy House
- Holiday Inn Express Niagara-On-The-Lake
- In Elegance
- Luisa's Suite Retreat
- Lulu's Bed & Breakfast
- Moffat Inn
- Oban Inn
- Old Town Country Landing
- Orchid Inn and Ginger Restaurant
- Pillar and Post Inn & Spa
- Prince of Wales
- Queen Regent Bed & Breakfast
- Queens Landing
- Residence & Conference Centre - Niagara-On-The-Lake
- Riverbend Inn and Vineyard
- School House Bed and Breakfast C.1859
- Serendipity Bed and Breakfast
- Somerset - A Private Retreat
- South Landing Inn
- The Charles Hotel
- The Old Bank House
- The Shaw Club Hotel
- The White House Boutique B&B
- Vineyard Villa Bed & Breakfast
- WeatherPine Inn
- White Oaks Resort & Spa
- Williams Gate Bed & Breakfast Private Suites
- Woodbourne Inn